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Cross-amplification and characterization of microsatellite loci for the Neotropical orchid genus Epidendrum Genet. Mol. Biol.
Pinheiro,Fábio; Palma-Silva,Clarisse; Barros,Fábio de; Cozzolino,Salvatore.
In this study we tested the cross-amplification of 33 microsatellite loci previously developed for two closely related Neotropical orchid genera (Epidendrum and Laelia). A set of ten loci were polymorphic across five examined species (20 individuals each) with 2 to 15 alleles per locus. The mean expected and observed heterozygosity (average across species) ranged from 0.34 to 0.82 and from 0.27 to 0.85, respectively. In addition we tested all loci in 35 species representative of the genus Epidendrum. Of these, 26 loci showed successful amplification. Cross-application of these loci represent a potential source of co-dominant markers for evolutionary, ecological and conservation studies in this important orchid genus.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Epidendrum; Orchidaceae; Short tandem repeat; Cross-amplification.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200021
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Morphometric analysis of the Brasiliorchis picta complex (Orchidaceae) Rev. Bras. Bot.
Pinheiro,Fábio; Barros,Fábio de.
One of the largest genera of Orchidaceae in the Neotropics with about 450 species, Maxillaria presents several taxonomic uncertainties about its generic circumscription and the delimitation of species groups, mainly due to the large variability of some species. The present study aims at verifying the morphological variation and species delimitation in the Brasiliorchis picta complex, a recent new genus derived from Maxillaria, using morphometric multivariate analysis. A total of 340 specimens belonging to six species (B. chrysantha (Barb. Rodr.) R.B. Singer, S. Koehler & Carnevali, B. gracilis (Lodd.) R.B. Singer, S. Koehler & Carnevali, B. marginata (Lindl.) R.B. Singer, S. Koehler & Carnevali, B. picta (Hook.) R. Singer, S....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Morphometry; Multivariate analysis; Species complex; Taxonomy.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042009000100003
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